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Image Search Results


Anlotinib inhibits the expression of inflammatory factors in tumor tissues and promotes tumor cell apoptosis. (A) Representative photographs of H&E staining and immunofluorescence staining at low magnification for the four treatment groups(n=5)(Scarl bar: 100um). (B) Representative images of immunofluorescence staining was used to detect the expression of α-SMA and IL-6 in the four treatment groups(n=5)(Scarl bar: 20um). (C) Representative images of immunofluorescence staining was used to detect the expression of α-SMA and IL-8 in the four treatment groups (n=5)(Scarl bar: 20um). (D) Representative images of immunofluorescence staining was used to detect the expression of α-SMA and VEGFA in the four treatment groups(n=5) (Scarl bar: 20um). (E) Representative bands for western blot analysis of Akt, Erk, Caspase3, and Cleaved Caspase3 in tumor tissues from the four treatment groups(n=3). *P<0.05, **P<0.01, ***P<0.001. Compared with the control group.

Journal: Frontiers in Oncology

Article Title: Anlotinib inhibits cervical cancer cell proliferation and invasion by suppressing cytokine secretion in activated cancer-associated fibroblasts

doi: 10.3389/fonc.2024.1412660

Figure Lengend Snippet: Anlotinib inhibits the expression of inflammatory factors in tumor tissues and promotes tumor cell apoptosis. (A) Representative photographs of H&E staining and immunofluorescence staining at low magnification for the four treatment groups(n=5)(Scarl bar: 100um). (B) Representative images of immunofluorescence staining was used to detect the expression of α-SMA and IL-6 in the four treatment groups(n=5)(Scarl bar: 20um). (C) Representative images of immunofluorescence staining was used to detect the expression of α-SMA and IL-8 in the four treatment groups (n=5)(Scarl bar: 20um). (D) Representative images of immunofluorescence staining was used to detect the expression of α-SMA and VEGFA in the four treatment groups(n=5) (Scarl bar: 20um). (E) Representative bands for western blot analysis of Akt, Erk, Caspase3, and Cleaved Caspase3 in tumor tissues from the four treatment groups(n=3). *P<0.05, **P<0.01, ***P<0.001. Compared with the control group.

Article Snippet: The primary antibodies used in this study were anti-α-SMA mouse mAb (A2547, 1:1000, Sigma-Aldrich, St. Louis, MO, USA), phosphorylated Stat 3(p-Stat3) rabbit mAb (#9145, 1:1000, Cell Signaling Technology, MA, USA), Stat 3 mouse mAb (#9139S, 1:1000, Cell Signaling Technology), phosphorylated janus kinase-2 rabbit mAb (p-Jak2) (#3771,1: 1000, Cell Signaling Technology, Massachusetts, USA), Jak2 rabbit mAb(#3230, 1: 1000, Cell Signaling Technology), Fap rabbit mAb (#52818S, 1:1000, Cell Signaling Technology), Akt rabbit mAb(#4691, 1:1000, Cell Signaling Technology), Erk rabbit mAb(#4695, 1:1000, Cell Signaling Technology), Caspase3 rabbit mAb(#9662, 1:1000, Cell Signaling Technology), Cleaved Caspase3 rabbit mAb(#9664, 1:1000, Cell Signaling Technology) and anti-Gapdh mouse mAb(#2118, 1:1,000,Cell Signaling Technology) were used as loading controls.

Techniques: Expressing, Staining, Immunofluorescence, Western Blot, Control